High performance SPR

Technical Documents

High-performance Surface Plasmon Resonance instrument (SPR)

We introduced the latest SPR instrument, Sierra SPR-24.

＜1＞High-performance surface plasmon resonance instrument (SPR)

The high-performance surface plasmon resonance instrument (SPR) allows label-free, real-time detection of intermolecular interaction and provides kinetics information, such as binding affinity or binding/dissociation kinetics. SPR is useful for interaction analysis of a wide range of molecules including small molecules, peptides, nucleic acids, and proteins.

＜2＞Features of high-performance surface plasmon resonance instrument (SPR)

High-throughput

  • Equipped with 8 channels having 3 sensor spots, and 24 sensor spots in total are available with a sensor chip. Ligand immobilization and analyte injection can be conducted individually per spot. Up to 24 different ligands can be screened at once.
  • Since the 24 sensor spots can be used completely individually, comparison of analysis conditions is easily conducted.

High-sensitivity

  • Enables robust measurement of interaction with small molecule as fragments, compounds, and others.
  • Baseline noise＜0.02 RU（RMS）

＜3＞Analysis example utilized numerous sensor spots

SICK（Single Injection Cycle Kinetics）method
This method realizes a speedy kinetics analysis by injecting analyte solutions of different concentrations to numerous sensor spots where the same ligands are immobilized.

＜4＞Application

  • Screening and kinetics analysis of small molecules for target proteins
  • Screening and characterization of biopharmaceuticals
  • Various intermolecular interaction analyses of compounds, peptides, nucleic acids, proteins, and sugars
  • Comprehensive intermolecular interaction analysis combined with other methods, such as ITC, NMR, MS, CD, and high-speed AMF