Tissue Engineering & Regenerative Medicine

Comprehensive analysis of medium for effective cell culture
Qualitative and quantitative analyses of medium components are very important for quality improvement and quality assurance since medium components have an great effect on efficiency of cell culture. TechData_P01410E (PDF:263KB)
Analysis of Inorganic Elements in Media using ICP-MS and ICP-AES
Media are widely used in the manufacturing of biotech products such as biopharmaceuticals or regenerative medicines. For evaluating the characteristics of media and the purification schemes used in manufacturing process, an analysis of inorganic elements can be performed using ICP mass spectrometry (ICP-QMS and ICP-SFMS) and ICP atomic emission spectrometry (ICP-AES). TechData_P01514E (PDF:232KB)
Deep Proteomics
Here we demonstrate a new LC-MS/MS analysis method for peptide mixtures with high resolution by combining ion exchange chromatography and C18 long monolith column chromatography. Analysis of standard cultured cells resulted that more than 6000 proteins were identified at a quantifiable level. TechData_P01798E (PDF:396KB)
Measurement of number of low molecular weight compounds labeled to proteins
In the field of diagnosis, etc., it is often required to evaluate how many compounds are covalently labeled per molecule of the protein. Here we introduce examples of how many FITC dyes were labeled in the antibody. This measurement is also applied to analysis of antibody-drug conjugates (ADCs). TechData_P01766E (PDF:501KB)
Evaluation of in-plane Homogeneity of Thickness and Mechanical Property of Collagen as a Scaffolding Member Using TOF-SIMS and AFM in Fluid
To evaluate the thickness uniformity and mechanical property of a scaffolding member is important because those properties affect the efficiency of cell culture remarkably. TOF-SIMS and AFM in fluid enable us to evaluate in-plane homogeneity of a scaffolding member such as collagen. TechData_P01458E (PDF:3,361KB)